Create regions which represents the plane(s) where the mold cavity and core meet, using the (Geometry tab > Create panel > Regions) and (Geometry tab > Create panel > Regions > Hole) tools. The region(s) should completely surround the part and have a hole where the part is.
Select all the regions you just created.
Click (Geometry tab > Properties panel > Assign).
Click New > Parting surface. The Parting Surface dialog appears.
Specify the interface conductance across the parting plane and click OK. The interface conductance depends on the mold material, the contact pressure, whether there is air or grease between the mold cavity and core, and the thickness of the gap.
Mesh the new regions by clicking (Mesh tab > Mesh panel > Generate Mesh).
If you have not done so already, model the external mold surface. Leave a small gap between the edge of the parting plane and the mold surface to avoid element intersection problems.
Tip: A parting plane can be placed at any location within the mold to view the thermal distribution across that plane.