FAQ: Why Do I Get The "File may be read-only or in use." Message When Digitally Signing an AutoLISP File?
The "File may be read-only or in use." error message is displayed when a digital signature can't be attached to a file with the Attach Digital Signatures (AcSignApply.exe) tool.
Some of the reasons for the error message being displayed are:
The file is open or locked in memory by an application or the operating system.
The file is located on a drive that you don't have write access.
The digital certificate you selected might be corrupt.