If you populate a collection using an expression, then add nodes to the scene that match this expression; for example, by creating objects or referencing objects, your active layer may need to be updated to reflect the newly added members.
When the scene changes, a red border appears around the layer visibility icon
to reflect that your layer membership may have changed. Click the icon to update your layer accordingly.
To automatically update your render setup nodes each time your scene changes, enable Update active layer when scene changes in the Options menu of the Render Setup editor.
Note: Performance of your scene may be affected when this option is enabled.