When the scene changes, a red border may appear around the layer visibility icon to reflect that your layer membership may have changed. Click the icon to update your layer accordingly.
If you populate a collection using an expression, then add nodes to the scene that match this expression; for example, by creating objects or referencing objects, your active layer may need to be updated to reflect the newly added members. In addition, when you create an override, or import a .json template, your active layer may also need to be refreshed. Click this icon when the red border appears.